Abstract
The collection of Pinocchia daecheonga sp. nov. was performed at the Daecheong Lake in Korea, five strains of which with four clones were investigated through light microscopy, transmission electron microscopy and molecular data comprising the 16S rRNA to 23S rRNA gene. P. daecheonga differed from type species P. polymorpha by absence of the polar aerotopes. Investigated stains and four clones of P. daecheonga turned out to be a sister clade to the P. polymorpha according to the phylogenetic analysis of 16S rRNA gene. In addition, the Pinocchia was clustered with the family Leptolyngbyaceae members, genera Leptothoe and Leptoelongatus. 16S–23S rRNA intergenic transcribed spacer (ITS) region of P. daecheonga was found to be substantially distinct to P. polymorpha in terms of the secondary structure and nucleotide sequence composition, which concludes that the Pinocchia daecheonga isolated from the Daecheong Lake is a unique species due to differences in morphology and genetic traits compared to the relative P. polymorpha.
References
Anagnostidis, K. & Komárek, J. (1988) Modern approach to the classification system of cyanophytes. 3. Oscillatoriales. Archiv für Hydrobiologie, Supplement 80: 327–472.
Becerra-Absalón, I., Johansen, J.R., Muñoz-Martín, M.A. & Montejano, G. (2018) Chroakolemma gen. nov. (Leptolyngbyaceae, Cyanobacteria) from soil biocrusts in the semi-desert Central Region of Mexico. Phytotaxa 367: 201–218. https://doi.org/10.11646/phytotaxa.367.3.1
Casamatta, D.A., Johansen, J.R., Vis, M.L. & Broadwater, S.T. (2005) Molecular and morphological characterization of ten polar and near-polar strains within the Oscillatoriales (Cyanobacteria). Journal of Phycology 41: 421–438. https://doi.org/10.1111/j.1529-8817.2005.04062.x
Chakraborty, S., Maruthanayagam, V., Achari, A., Pramanik, A., Jaisankar, P. & Mukherjee, J. (2019) Euryhalinema mangrovii gen. nov., sp. nov. and Leptoelongatus litoralis gen. nov., sp. nov. (Leptolyngbyaceae) isolated from an Indian mangrove forest. Phytotaxa 422: 58–74. https://doi.org/10.11646/phytotaxa.422.1.4
Chun, S.-J., Cui, Y., Jin, C., Cho, A.-R., Wong, S.-K., Lee, H.-G., Oh, H.-M. & Ahn, C.-Y. (2020) Paraconexibacter algicola gen. nov., sp. nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam. nov. in the order Solirubrobacterales. International Journal of Systematic and Evolutionary Microbiology 70: 915–922. https://doi.org/10.1099/ijsem.0.003846
Dadheech, P.K., Mahmoud, H., Kotut, K. & Krienitz, L. (2012) Haloleptolyngbya alcalis gen. et sp. nov., a new filamentous cyanobacterium from the soda lake Nakuru, Kenya. Hydrobiologia 691: 269–283. https://doi.org/10.1007/s10750-012-1080-6
Davydov, D., Shalygin, S. & Vilnet, A. (2020) New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago. Phytotaxa 442: 61–79. https://doi.org/10.11646/phytotaxa.442.2.2
Dvo?ák, P., Jahodá?ová, E., Hašler, P., Gusev, E. & Poulí?ková, A. (2015) A new tropical cyanobacterium Pinocchia polymorpha gen. et sp. nov. derived from the genus Pseudanabaena. Fottea 15: 113–120. https://doi.org/10.5507/fot.2015.010
Engene, N. & Tronholm, A. (2019) Moorena gen. nov., a valid name for “Moorea Engene & al.” nom. inval. Oscillatoriaceae, Cyanobacteria). Notulae Algarum 122: 1–2.
Gomont, M. (1892) Monographie des Oscillariées (Nostocacées Homocystées). Deuxième partie. -Lyngbyées. Annales des Sciences Naturelles, Botanique, Série 7 15: 263–368; 16: 91–264.
Guiry, M.D. & Guiry, G.M. (2021) AlgaeBase. World-wide electronic publication. National University of Ireland, Galway. Available from: http://www.algaebase.org/ (accessed 1 February 2021)
Hall, T.A. (1999) BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposia Series 41: 95–98.
Hrouzek, P., Lukešová, A., Mareš, J. & Ventura, S. (2013) Description of the cyanobacterial genus Desmonostoc gen. nov. including D. muscorum comb. nov. as a distinct, phylogenetically coherent taxon related to the genus Nostoc. Fottea 13: 201–213. http://dx.doi.org/10.5507/fot.2013.016
Jahodá?ová, E., Dvo?ák, P., Hašler, P., Holušová, K. & Poulí?ková, A. (2017) Elainella gen. nov.: a new tropical cyanobacterium characterized using a complex genomic approach. European Journal of Phycology 53: 39–51. https://doi.org/10.1080/09670262.2017.1362591
Johansen, J.R., Kovacik, L., Casamatta, D.A., Fu?iková, K. & Kaštovský, J. (2011) Utility of 16S–23S ITS sequence and secondary structure for recognition of intrageneric and intergeneric limits within cyanobacterial taxa: Leptolyngbya corticola sp. nov. (Pseudanabanaceae, Cyanobacteria). Nova Hedwigia 92: 283–302. https://doi.org/10.1127/0029-5035/2011/0092-0283
Kim, J.-H., Jeong, M.S., Kim, D.-Y., Her, S. & Wie, M.-B. (2015) Zinc oxide nanoparticles induce lipoxygenase-mediated apoptosis and necrosis in human neuroblastoma SH-SY5Y cells. Neurochemistry International 90: 204–214. https://doi.org/10.1016/j.neuint.2015.09.002
Komárek, J. (2016) A polyphasic approach for the taxonomy of cyanobacteria: principles and applications. European Journal of Phycology 51: 346–353. https://doi.org/10.1080/09670262.2016.1163738
Komárek, J. (2018) Several problems of the polyphasic approach in the modern cyanobacterial system. Hydrobiologia 811: 7–17. https://doi.org/10.1007/s10750-017-3379-9
Komárek, J. & Anagnostidis, K. (2005) Cyanoprokaryota. 2. Teil: Oscillatoriales. In: Büdel, B., Krienitz, L., Gärtner, G. & Schagerl, M. (Eds.) Sü?wasserflora von Mitteleuropa, vol. 19/2. Elsevier, Spektrum, Heidelberg, pp. 1–759.
Komárek, J. & Lukavsky, J. (1988) Arthronema, a new cyanophyte genus from Afro-Asian deserts. Archiv für Hydrobiologie, Supplement 80: 249–267.
Komárek, J., Kaštovský, J., Mareš, J. & Johansen, J.R. (2014) Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia 86: 295–335.
Konstantinou, D., Voultsiadou, E., Panteris, E., Zervou, S.K., Hiskia, A. & Gkelis, S. (2019) Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology 55: 882–897. https://doi.org/10.1111/jpy.12866
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
Lauterborn, H. (1915) Die sapropelische Lebewelt. Ein Beitrag zur biologie des Faulschlamms natürlicher Gewässer. Verhandlungen des Naturhistorischen-Medizinischen Vereins zu Heidelberg, 13: 395–481.
Lee, N.-J., Seo, Y.-S., Ki, J.-S. & Lee, O.-M. (2019) A study of newly recorded genus and species for aerial cyanobacteria Wilmottia murrayi (Oscillatoriales, Cyanobacteria) in Korea. Korean Journal of Environmental Biology 37: 260–267. https://doi.org/10.11626/KJEB.2019.37.3.260
Lee, N-J., Seo, Y., Ki, J.-S. & Lee, O.-M. (2020) Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea. Phytotaxa 447: 237–251. https://doi.org/10.11646/phytotaxa.447.4.2
Li, X. & Li, R. (2016) Limnolyngbya circumcreta gen. & comb. nov. (Synechococcales, Cyanobacteria) with three geographical (provincial) genotypes in China. Phycologia 55: 478–491. https://doi.org/10.2216/15-149.1
Mai, T., Johansen, J.R., Pietrasiak, N., Bohunická, M. & Martin, M.P. (2018) Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species. Phytotaxa 365: 1–59. https://doi.org/10.11646/phytotaxa.365.1.1
Miscoe, L.H., Johansen, J.R., Vaccarino, M.A., Pietrasiak, N. & Sherwood, A.R. (2016) Novel cyanobacteria from caves on Kauai, Hawaii. Bibliotheca Phycologica 120: 75–152.
Osario-Santos, K., Pietrasiak, N., Bohunická, M., Miscoe, L.H., Ková?ik, L., Martin, M.P & Johansen, J.R. (2014) Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria): taxonomically recognizing cryptic diversification. European Journal of Phycology 49: 450–470. https://doi.org/10.1080/09670262.2014.976843
Page, R.D.M. (1996) TreeView: An application to display phylogenetic trees on personal computers. Bioinformatics 12: 357–358.
Perkerson III, R.B., Johansen, J.R., Ková?ik, L., Brand, J., Kaštovský, J. & Casamatta, D.A. (2011) A unique pseudanabaenalean (Cyanobacteria) genus Nodosilinea gen. nov. based on morphological and molecular data. Journal of Phycology 47: 1397–1412. https://doi.org/10.1111/j.1529-8817.2011.01077.x
Pietrasiak, N., Osorio-Santos, K., Shalygin, S., Martin, M.P. & Johansen, J.R. (2019) When is a lineage a species? a case study in Myxacorys gen. nov. (Synechococcales: Cyanobacteria) with the description of two new species from the Americas. Journal of Phycology 55: 976–996. https://doi.org/10.1111/jpy.12897
Raabová, L., Kovacik, L., Elster, J. & Strunecký, O. (2019) Review of the genus Phormidesmis (Cyanobacteria) based on environmental, morphological, and molecular data with description of a new genus Leptodesmis. Phytotaxa 395: 1–16. https://doi.org/10.11646/phytotaxa.395.1.1
Richards, E., Reichardt, M. & Rogers, S. (2003) Preparation of genomic DNA from plant tissue. In: Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (Eds.) Current protocols in molecular biology. John Wiley and Sons, Inc., New York, pp. 2.3.1–2.3.7.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inferenceunder mixed models. Bioinformatics 19: 1572–1574. http://dx.doi.org/10.1093/bioinformatics/btg180
Siegesmund, M.A., Johansen, J.R., Karsten, U. & Friedl, T. (2008) Coleofasciculus gen. nov. (Cyanobacteria): morphological and molecular criteria for revision of the genus Microcoleus Gomont. Journal of Phycology 44: 1572–1585. https://doi.org/10.1111/j.1529-8817.2008.00604.x
Song, G., Jiang, Y. & Li, R. (2015) Scytolyngbya timoleontis, gen. et sp. nov. (Leptolyngbyaceae, Cyanobacteria): a novel false branching cyanobacteria from China. Phytotaxa 224: 72–84. https://doi.org/10.11646/phytotaxa.224.1.5
Stackebrandt, E. & Goebel, B.M. (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic Bacteriology 44: 846–849.
Stanier, R.Y., Kunisawa, R., Mandel, M. & Cohenbaz, G. (1971) Purification and properties of unicellular blue-green algae (Order Chroococcales). Bacteriological Reviews 35: 171–205.
Taton, A., Wilmotte, A., Šmarda, J., Elster, J. & Komárek, J. (2011) Plectolyngbya hodgsonii: a novel filamentous cyanobacterium from Antarctic lakes. Polar Biology 34: 181–191. http://dx.doi.org/10.1007/s00300-010-0868-y
Vaz, M.G.M.V., Bonaldo-Genuário, D., Dini-Andreote, A.P., Silva-Malone, C.F., Sant’-Anna, C.L., Barbiero, L. & Fátima-Fiore, M. (2015) Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline–alkaline lakes. International Journal of Systematic and Evolutionary Microbiology 65: 298–308. https://doi.org/10.1099/ijs.0.070110-0
Zammit, G., Billi, D. & Albertano, P. (2012) The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales, Cyanophyceae) gen. et sp. nov.: a cytomorphological and molecular description. European Journal of Phycology 47: 341–354. http://dx.doi.org/ 10.1080/09670262.2012.717106
Zuker, M. (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research 31: 3406–3415. http://dx.doi.org/10.1093/nar/gkg595
Becerra-Absalón, I., Johansen, J.R., Muñoz-Martín, M.A. & Montejano, G. (2018) Chroakolemma gen. nov. (Leptolyngbyaceae, Cyanobacteria) from soil biocrusts in the semi-desert Central Region of Mexico. Phytotaxa 367: 201–218. https://doi.org/10.11646/phytotaxa.367.3.1
Casamatta, D.A., Johansen, J.R., Vis, M.L. & Broadwater, S.T. (2005) Molecular and morphological characterization of ten polar and near-polar strains within the Oscillatoriales (Cyanobacteria). Journal of Phycology 41: 421–438. https://doi.org/10.1111/j.1529-8817.2005.04062.x
Chakraborty, S., Maruthanayagam, V., Achari, A., Pramanik, A., Jaisankar, P. & Mukherjee, J. (2019) Euryhalinema mangrovii gen. nov., sp. nov. and Leptoelongatus litoralis gen. nov., sp. nov. (Leptolyngbyaceae) isolated from an Indian mangrove forest. Phytotaxa 422: 58–74. https://doi.org/10.11646/phytotaxa.422.1.4
Chun, S.-J., Cui, Y., Jin, C., Cho, A.-R., Wong, S.-K., Lee, H.-G., Oh, H.-M. & Ahn, C.-Y. (2020) Paraconexibacter algicola gen. nov., sp. nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam. nov. in the order Solirubrobacterales. International Journal of Systematic and Evolutionary Microbiology 70: 915–922. https://doi.org/10.1099/ijsem.0.003846
Dadheech, P.K., Mahmoud, H., Kotut, K. & Krienitz, L. (2012) Haloleptolyngbya alcalis gen. et sp. nov., a new filamentous cyanobacterium from the soda lake Nakuru, Kenya. Hydrobiologia 691: 269–283. https://doi.org/10.1007/s10750-012-1080-6
Davydov, D., Shalygin, S. & Vilnet, A. (2020) New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago. Phytotaxa 442: 61–79. https://doi.org/10.11646/phytotaxa.442.2.2
Dvo?ák, P., Jahodá?ová, E., Hašler, P., Gusev, E. & Poulí?ková, A. (2015) A new tropical cyanobacterium Pinocchia polymorpha gen. et sp. nov. derived from the genus Pseudanabaena. Fottea 15: 113–120. https://doi.org/10.5507/fot.2015.010
Engene, N. & Tronholm, A. (2019) Moorena gen. nov., a valid name for “Moorea Engene & al.” nom. inval. Oscillatoriaceae, Cyanobacteria). Notulae Algarum 122: 1–2.
Gomont, M. (1892) Monographie des Oscillariées (Nostocacées Homocystées). Deuxième partie. -Lyngbyées. Annales des Sciences Naturelles, Botanique, Série 7 15: 263–368; 16: 91–264.
Guiry, M.D. & Guiry, G.M. (2021) AlgaeBase. World-wide electronic publication. National University of Ireland, Galway. Available from: http://www.algaebase.org/ (accessed 1 February 2021)
Hall, T.A. (1999) BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposia Series 41: 95–98.
Hrouzek, P., Lukešová, A., Mareš, J. & Ventura, S. (2013) Description of the cyanobacterial genus Desmonostoc gen. nov. including D. muscorum comb. nov. as a distinct, phylogenetically coherent taxon related to the genus Nostoc. Fottea 13: 201–213. http://dx.doi.org/10.5507/fot.2013.016
Jahodá?ová, E., Dvo?ák, P., Hašler, P., Holušová, K. & Poulí?ková, A. (2017) Elainella gen. nov.: a new tropical cyanobacterium characterized using a complex genomic approach. European Journal of Phycology 53: 39–51. https://doi.org/10.1080/09670262.2017.1362591
Johansen, J.R., Kovacik, L., Casamatta, D.A., Fu?iková, K. & Kaštovský, J. (2011) Utility of 16S–23S ITS sequence and secondary structure for recognition of intrageneric and intergeneric limits within cyanobacterial taxa: Leptolyngbya corticola sp. nov. (Pseudanabanaceae, Cyanobacteria). Nova Hedwigia 92: 283–302. https://doi.org/10.1127/0029-5035/2011/0092-0283
Kim, J.-H., Jeong, M.S., Kim, D.-Y., Her, S. & Wie, M.-B. (2015) Zinc oxide nanoparticles induce lipoxygenase-mediated apoptosis and necrosis in human neuroblastoma SH-SY5Y cells. Neurochemistry International 90: 204–214. https://doi.org/10.1016/j.neuint.2015.09.002
Komárek, J. (2016) A polyphasic approach for the taxonomy of cyanobacteria: principles and applications. European Journal of Phycology 51: 346–353. https://doi.org/10.1080/09670262.2016.1163738
Komárek, J. (2018) Several problems of the polyphasic approach in the modern cyanobacterial system. Hydrobiologia 811: 7–17. https://doi.org/10.1007/s10750-017-3379-9
Komárek, J. & Anagnostidis, K. (2005) Cyanoprokaryota. 2. Teil: Oscillatoriales. In: Büdel, B., Krienitz, L., Gärtner, G. & Schagerl, M. (Eds.) Sü?wasserflora von Mitteleuropa, vol. 19/2. Elsevier, Spektrum, Heidelberg, pp. 1–759.
Komárek, J. & Lukavsky, J. (1988) Arthronema, a new cyanophyte genus from Afro-Asian deserts. Archiv für Hydrobiologie, Supplement 80: 249–267.
Komárek, J., Kaštovský, J., Mareš, J. & Johansen, J.R. (2014) Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia 86: 295–335.
Konstantinou, D., Voultsiadou, E., Panteris, E., Zervou, S.K., Hiskia, A. & Gkelis, S. (2019) Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology 55: 882–897. https://doi.org/10.1111/jpy.12866
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870–1874. https://doi.org/10.1093/molbev/msw054
Lauterborn, H. (1915) Die sapropelische Lebewelt. Ein Beitrag zur biologie des Faulschlamms natürlicher Gewässer. Verhandlungen des Naturhistorischen-Medizinischen Vereins zu Heidelberg, 13: 395–481.
Lee, N.-J., Seo, Y.-S., Ki, J.-S. & Lee, O.-M. (2019) A study of newly recorded genus and species for aerial cyanobacteria Wilmottia murrayi (Oscillatoriales, Cyanobacteria) in Korea. Korean Journal of Environmental Biology 37: 260–267. https://doi.org/10.11626/KJEB.2019.37.3.260
Lee, N-J., Seo, Y., Ki, J.-S. & Lee, O.-M. (2020) Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea. Phytotaxa 447: 237–251. https://doi.org/10.11646/phytotaxa.447.4.2
Li, X. & Li, R. (2016) Limnolyngbya circumcreta gen. & comb. nov. (Synechococcales, Cyanobacteria) with three geographical (provincial) genotypes in China. Phycologia 55: 478–491. https://doi.org/10.2216/15-149.1
Mai, T., Johansen, J.R., Pietrasiak, N., Bohunická, M. & Martin, M.P. (2018) Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species. Phytotaxa 365: 1–59. https://doi.org/10.11646/phytotaxa.365.1.1
Miscoe, L.H., Johansen, J.R., Vaccarino, M.A., Pietrasiak, N. & Sherwood, A.R. (2016) Novel cyanobacteria from caves on Kauai, Hawaii. Bibliotheca Phycologica 120: 75–152.
Osario-Santos, K., Pietrasiak, N., Bohunická, M., Miscoe, L.H., Ková?ik, L., Martin, M.P & Johansen, J.R. (2014) Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria): taxonomically recognizing cryptic diversification. European Journal of Phycology 49: 450–470. https://doi.org/10.1080/09670262.2014.976843
Page, R.D.M. (1996) TreeView: An application to display phylogenetic trees on personal computers. Bioinformatics 12: 357–358.
Perkerson III, R.B., Johansen, J.R., Ková?ik, L., Brand, J., Kaštovský, J. & Casamatta, D.A. (2011) A unique pseudanabaenalean (Cyanobacteria) genus Nodosilinea gen. nov. based on morphological and molecular data. Journal of Phycology 47: 1397–1412. https://doi.org/10.1111/j.1529-8817.2011.01077.x
Pietrasiak, N., Osorio-Santos, K., Shalygin, S., Martin, M.P. & Johansen, J.R. (2019) When is a lineage a species? a case study in Myxacorys gen. nov. (Synechococcales: Cyanobacteria) with the description of two new species from the Americas. Journal of Phycology 55: 976–996. https://doi.org/10.1111/jpy.12897
Raabová, L., Kovacik, L., Elster, J. & Strunecký, O. (2019) Review of the genus Phormidesmis (Cyanobacteria) based on environmental, morphological, and molecular data with description of a new genus Leptodesmis. Phytotaxa 395: 1–16. https://doi.org/10.11646/phytotaxa.395.1.1
Richards, E., Reichardt, M. & Rogers, S. (2003) Preparation of genomic DNA from plant tissue. In: Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (Eds.) Current protocols in molecular biology. John Wiley and Sons, Inc., New York, pp. 2.3.1–2.3.7.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inferenceunder mixed models. Bioinformatics 19: 1572–1574. http://dx.doi.org/10.1093/bioinformatics/btg180
Siegesmund, M.A., Johansen, J.R., Karsten, U. & Friedl, T. (2008) Coleofasciculus gen. nov. (Cyanobacteria): morphological and molecular criteria for revision of the genus Microcoleus Gomont. Journal of Phycology 44: 1572–1585. https://doi.org/10.1111/j.1529-8817.2008.00604.x
Song, G., Jiang, Y. & Li, R. (2015) Scytolyngbya timoleontis, gen. et sp. nov. (Leptolyngbyaceae, Cyanobacteria): a novel false branching cyanobacteria from China. Phytotaxa 224: 72–84. https://doi.org/10.11646/phytotaxa.224.1.5
Stackebrandt, E. & Goebel, B.M. (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic Bacteriology 44: 846–849.
Stanier, R.Y., Kunisawa, R., Mandel, M. & Cohenbaz, G. (1971) Purification and properties of unicellular blue-green algae (Order Chroococcales). Bacteriological Reviews 35: 171–205.
Taton, A., Wilmotte, A., Šmarda, J., Elster, J. & Komárek, J. (2011) Plectolyngbya hodgsonii: a novel filamentous cyanobacterium from Antarctic lakes. Polar Biology 34: 181–191. http://dx.doi.org/10.1007/s00300-010-0868-y
Vaz, M.G.M.V., Bonaldo-Genuário, D., Dini-Andreote, A.P., Silva-Malone, C.F., Sant’-Anna, C.L., Barbiero, L. & Fátima-Fiore, M. (2015) Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline–alkaline lakes. International Journal of Systematic and Evolutionary Microbiology 65: 298–308. https://doi.org/10.1099/ijs.0.070110-0
Zammit, G., Billi, D. & Albertano, P. (2012) The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales, Cyanophyceae) gen. et sp. nov.: a cytomorphological and molecular description. European Journal of Phycology 47: 341–354. http://dx.doi.org/ 10.1080/09670262.2012.717106
Zuker, M. (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research 31: 3406–3415. http://dx.doi.org/10.1093/nar/gkg595