Abstract
During an investigation of ascomycetous fungi in Karst formations of the Asian region, three interesting taxa were found on dead aerial stems of woody hosts in Guizhou province, China. Their morphology assigned them as typical botryosphaeriaceous species. Phylogenetic analyses based on a combined DNA dataset of large subunit (LSU), internal transcribed spacer (ITS) and part of the translation elongation factor 1-α (tef1) gene confirmed their placement within Botryosphaeriaceae. In the phylogenetic tree, the three isolates formed a well-supported monotypic clade as a distinct lineage within the genus Sardiniella. Therefore, a new species Sardiniella guizhouensis sp. nov. is introduced to accommodate these taxa and detailed, illustrated descriptions of the asexual and sexual morphs are provided. This study reveals the first sexual morph of Sardiniella, which it is characterized by 2–4(–6)-spored asci with hyaline to brown, aseptate to 1-septate, ovate to subclavate ascospores.
References
<p>Ariyawansa, H.A., Hyde, K.D., Liu, J.K., Wu, S.P. & Liu, Z.Y. (2016) Additions to Karst Fungi 1: <em>Botryosphaeria minutispermatia</em> sp. nov., from Guizhou Province, China. <em>Phytotaxa</em> 275 (1): 35–44.</p>
<p> https://doi.org/10.11646/phytotaxa.275.1.4</p>
<p>Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. <em>Mycologia</em> 91: 553–556.</p>
<p> https://doi.org/10.1080/00275514.1999.12061051</p>
<p>Chen, Y.Y., Maharachchikumbura, S.S.N., Liu, J.K., Hyde, K.D., Nanayakkara, R.R., Zhu, G.S. & Liu, Z.Y. (2017) Fungi from Asian Karst formations I. <em>Pestalotiopsis photinicola</em> sp. nov., causing leaf spots of <em>Photinia serrulata</em>. <em>Mycosphere</em> 8: 103–110. https://doi.org/10.5943/mycosphere/8/1/9</p>
<p>Chen, Y.Y., Dissanayake, A.J., Liu, Z.Y. & Liu, J.K. (2020) Additions to Karst Fungi 4: <em>Botryosphaeria</em> spp. associated with woody hosts in Guizhou province, China including <em>B. guttulata</em> sp. nov. <em>Phytotaxa</em> 454 (3): 186–202.</p>
<p> https://doi.org/10.11646/phytotaxa.454.3.2</p>
<p>Crous, P.W., Slippers, B., Wingfield, M.J., Rheeder, J., Marasas, W.F.O., Philips, A.J.L., Alves, A., Burgess, T.I., Barber, P.A. & Groenewald, J.Z. (2006) Phylogenetic lineages in the Botryosphaeriaceae. <em>Studies in Mycology</em> 55: 235–253.</p>
<p> https://doi.org/10.3114/sim.55.1.235</p>
<p>Dissanayake, A.J., Phillips, A.J.L., Hyde, K.D. & Li, X.H. (2016) Botryosphaeriaceae: Current status of genera and species. <em>Mycosphere</em> 7: 1001–1073.</p>
<p> https://doi.org/10.5943/mycosphere/si/1b/13</p>
<p>Dissanayake, A.J., Chen, Y.Y. & Liu, J.K. (2020a) Unravelling <em>Diaporthe</em> species associated with woody hosts from Karst Formations (Guizhou) in China. <em>Journal of Fungi</em>, 6 (4) 251.https://doi:10.3390/jof6040251</p>
<p>Dissanayake, A.J., Bhunjun, C.S., Maharachchikumbura, S.S.N. & Liu, J.K. (2020b) Applied aspects of methods to infer phylogenetic relationships amongst fungi. <em>Mycosphere</em> 11: 2653–2677.https://doi.org/10.5943/mycosphere/11/1/18</p>
<p>Doilom, M., Shuttleworth, L.A., Roux, J., Chukeatirote, E. & Hyde, K.D. (2014) <em>Barriopsis tectonae</em> sp. nov. a new species of Botryosphaeriaceae from <em>Tectona grandis </em>(teak) in Thailand. <em>Phytotaxa</em> 176 (1): 81–91.</p>
<p> http://dx.doi.org/10.11646/phytotaxa.176.1.10</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.</p>
<p>Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. <em>Bioinformatics</em> 17: 754–755.https://doi.org/10.1093/bioinformatics/17.8.754</p>
<p>Hyde, K.D., Nilsson, R.H., Alias, S.A., Ariyawansa, H.A., Blair, J.E., Cai, L., de Cock, A.W.A.M., Dissanayake, A.J., Glockling, S.L., Goonasekara, I.D., Gorczak, M., Hahn, M., Jayawardena, R.S., van Kan, J.A.L., Laurence, M.H., Le´vesque, C.A., Li, X., Liu, J.K., Maharachchikumbura, S.S.N., Manamgoda, D.S., Martin, F.N., McKenzie, E.H.C., McTaggart. A.R., Mortimer, P.E., Nair, Julia Pawłowska, P.V.R., Rintoul, T.L., Shivas, R.G., Spies, C.F.J., Summerell, B.A., Taylor, P.W.J., Terhem, R.B., Udayanga, D., Vaghefi, N., Walther, G., Wilk, M., Wrzosek, M., Xu, J.C., Yan, J.Y. & Zhou, N. (2014) One stop shop: backbones trees for important phytopathogenic genera: I (2014). <em>Fungal Diversity</em> 67: 21–125https://doi.org/10.1007/s13225-014-0298-1</p>
<p>Hyde, K.D., Norphanphoun, C., Abreu, V.P., Bazzicalupo, A., Chethana, K.T., Clericuzio, M., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., He, M.Q., Hongsanan, S., Huang, S.K., Jayasiri, R.S., Jayawardena, R.S., Karunarathna, A., Konta, S., Kusˇan, I., Lee, H., Li, J., Lin, C.G., Liu, N.G., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Mapook, A., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Siedlecki, I., Soares, A.M., Tennakoon, D.S., Tian, Q., Tibpromma, S., Wanasinghe, D.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Abdel-Aziz, F.A., Li, W.J., Senanayake, I.C., Shang, Q.J., Daranagama, D.A., de Silva, N.I., Thambugala, K.M., Abdel-Wahab, M.A., Bahkali, A.H., Berbee, M.L., Boonmee, S., Bhat, D.J., Bulgakov, T.S., Buyck, B., Camporesi, E., Castan˜eda-Ruiz, R.F., Chomnunti, P., Doilom, M., Dovana, F., Gibertoni, T.B., Jadan, M., Jeewon, R., Jones, E.B.G., Kang, J.C., Karunarathna, S.C., Lim, Y.W., Liu, J.K., Liu, Z.Y., Plautz, H.L. Jr, Lumyong, S., Maharachchikumbura, S.S.N., Matocˇec, N., McKenzie, E.H.C., Mesˇic´, A., Miller, D., Pawlowska, J., Pereira, O.L., Promputtha, I., Romero, A.I., Ryvarden, L., Su, H.Y., Suetrong, S., Tkalcˇec, Z., Vizzini, A., Wen, T.C., Wisitrassameewong, K., Wrzosek, M., Xu, J.C., Zhao, Q., Zhao, R.L. & Mortimer, P.E. (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. <em>Fungal Diversity</em> 87: 1–235</p>
<p> https://doi.org/10.1007/s13225-017-0391-3</p>
<p>Index Fungorum (2021) [http://www.indexfungorum.org/Names/Names.asp]</p>
<p>Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., De Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. <em>Fungal Diversity</em> 74 (1): 3–18.</p>
<p> https://doi.org/10.1007/s13225-015-0351-8</p>
<p>Katoh, K. & Standley, K. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. <em>Molecular Biology & Evolution</em> 30: 772–780.</p>
<p> https://doi.org/10.1093/molbev/mst010</p>
<p>Kishino, H. & Hasegawa, M. (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. <em>Journal of Molecular Evolution</em> 29: 170–179.</p>
<p> https://doi.org/10.1007/BF02100115</p>
<p>Linaldeddu, B.T., Alves, A. & Phillips, A.J.L. (2016) <em>Sardiniella urbana</em> gen. et sp. nov., a new member of the Botryosphaeriaceae isolated from declining <em>Celtis australis</em> trees in Sardinian streetscapes. <em>Mycosphere</em> 7: 893–905.https://doi.org/10.5943/mycosphere/si/1b/5</p>
<p>Liu, J.K., Chomnunti, P., Cai, L., Phookamsak, R., Chukeatirote, E., Jones, E.B.G., Moslem, M. & Hyde, K.D. (2010) Phylogeny and morphology of <em>Neodeightonia palmicola</em> sp. nov. from palms. <em>Sydowia</em> 62: 261−276.</p>
<p>Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansa, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, J.D., Romero, A.I., Zhuang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., Ko Ko, T.W., Zhao, Y.C., Wang, Y. & Hyde, K.D. (2012) Towards a natural classification of Botryosphaeriales. <em>Fungal Diversity</em> 57: 149–210.</p>
<p> https://doi.org/10.1007/s13225-012-0207-4</p>
<p>Nylander, J.A.A. (2004) MrModeltest v2.2. Program distributed by the author: 2. Evolutionary Biology Centre, Uppsala University, 1–2.</p>
<p>Phillips, A.J.L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M.J., Groenewald, J.Z. & Crous, P.W. (2013) The Botryosphaeriaceae: genera and species known from culture. <em>Studies in Mycology</em> 76: 51–167.</p>
<p> https://doi.org/10.3114/sim0021</p>
<p>Phillips, A.J.L., Hyde, K.D., Alves A. & Liu, J.K (2019) Families in Botryosphaeriales: a phylogenetic, morphological and evolutionary perspective. <em>Fungal Diversity</em> 94: 1–22.</p>
<p> https://doi.org/10.1007/s13225-018-0416-6</p>
<p>Rayner, R.W. (1970) <em>A Mycological Colour Chart</em>. Commonwealth Mycological Institute, Kew, UK.</p>
<p>Silvestro, D. & Michalak, I. (2010) raxmlGUI: a graphical front-end for RAxML Program. Available from: https://sourceforge.net/ (accessed 1 August 2010)</p>
<p>Swofford, D.L. (2002) PAUP* 4.0: phylogenetic analysis using parsimony (* and other methods). Sinauer Associates, Sunderland.</p>
<p>Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. <em>J Bacteriol</em> 172: 4238–4246.https://doi.org/10.1128/JB.172.8.4238-4246.1990</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) <em>PCR protocols</em>: <em>A Guide to Methods and Applications</em>. Academic Press, New York, USA, pp. 315–322.</p>
<p> https://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Yang, T., Groenewald, J.Z., Cheewangkoon, R., Jami, F., Amdollahzadeh, J., Lombard, L. & Crous, P.W. (2017) Families, genera, and species of Botryosphaeriales. <em>Fungal Biology</em> 121: 322–346.</p>
<p> http://dx.doi.org/10.1016/j.funbio.2016.11.001</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Chen, Y.Y., Liu, Y.X. & Liu, Z.Y. (2017a) Two new species of <em>Dyfrolomyces</em> (Dyfrolomycetaceae, Dothideomycetes) from karst landforms. <em>Phytotaxa</em> 313 (3): 267–277.</p>
<p> https://doi.org/10.11646/phytotaxa.313.3.4</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Yang, W. & Liu, Z.Y. (2017b) Fungi from Asian Karst formations II. Two new species of <em>Occultibambusa</em> (Occultibambusaceae, Dothideomycetes) from karst landforms of China. <em>Mycosphere</em> 8: 550–559.</p>
<p> https://doi.org/10.5943/mycosphere/8/4/4</p>
<p>Zhang, J.F., Liu, J.K., Ran, H.Y., Khongphinitbunjong, K. & Liu, Z.Y. (2018) A new species and new record of <em>Lophiotrema</em> (Lophiotremataceae, Dothideomycetes) from karst landforms in southwest China. <em>Phytotaxa</em> 379 (2): 169–179.</p>
<p> https://doi.org/10.11646/phytotaxa.379.2.5</p>
<p>Zhang, J.F., Liu, J.K., Jeewon, R., Wanasinghe, D.N. & Liu, Z.Y. (2019) Fungi from Asian Karst formations III. Molecular and morphological characterization reveal new taxa in Phaeosphaeriaceae. <em>Mycosphere</em> 10: 202–220. https://doi.org/10.5943/mycosphere/10/1/3</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Ekanayaka, A.H. & Liu, Z.Y. (2020) Morpho-phylogenetic evidence reveals new species in <em>Rhytismataceae</em> (Rhytismatales, Leotiomycetes, Ascomycota) from Guizhou Province, China. <em>MycoKeys</em> 76: 81–106. https://doi.org/10.3897/mycokeys.76.58465</p>
<p>Zhang, W., Groenewald, J.Z., Lombard, L., Schumacher, R.K., Phillips, A.J.L. & Crous, P.W. (2021) Evaluating species in Botryosphaeriales. <em>Persoonia</em> 46: 63–115.</p>
<p> https://doi.org/10.3767/persoonia.2021.46.03</p>
<p> https://doi.org/10.11646/phytotaxa.275.1.4</p>
<p>Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. <em>Mycologia</em> 91: 553–556.</p>
<p> https://doi.org/10.1080/00275514.1999.12061051</p>
<p>Chen, Y.Y., Maharachchikumbura, S.S.N., Liu, J.K., Hyde, K.D., Nanayakkara, R.R., Zhu, G.S. & Liu, Z.Y. (2017) Fungi from Asian Karst formations I. <em>Pestalotiopsis photinicola</em> sp. nov., causing leaf spots of <em>Photinia serrulata</em>. <em>Mycosphere</em> 8: 103–110. https://doi.org/10.5943/mycosphere/8/1/9</p>
<p>Chen, Y.Y., Dissanayake, A.J., Liu, Z.Y. & Liu, J.K. (2020) Additions to Karst Fungi 4: <em>Botryosphaeria</em> spp. associated with woody hosts in Guizhou province, China including <em>B. guttulata</em> sp. nov. <em>Phytotaxa</em> 454 (3): 186–202.</p>
<p> https://doi.org/10.11646/phytotaxa.454.3.2</p>
<p>Crous, P.W., Slippers, B., Wingfield, M.J., Rheeder, J., Marasas, W.F.O., Philips, A.J.L., Alves, A., Burgess, T.I., Barber, P.A. & Groenewald, J.Z. (2006) Phylogenetic lineages in the Botryosphaeriaceae. <em>Studies in Mycology</em> 55: 235–253.</p>
<p> https://doi.org/10.3114/sim.55.1.235</p>
<p>Dissanayake, A.J., Phillips, A.J.L., Hyde, K.D. & Li, X.H. (2016) Botryosphaeriaceae: Current status of genera and species. <em>Mycosphere</em> 7: 1001–1073.</p>
<p> https://doi.org/10.5943/mycosphere/si/1b/13</p>
<p>Dissanayake, A.J., Chen, Y.Y. & Liu, J.K. (2020a) Unravelling <em>Diaporthe</em> species associated with woody hosts from Karst Formations (Guizhou) in China. <em>Journal of Fungi</em>, 6 (4) 251.https://doi:10.3390/jof6040251</p>
<p>Dissanayake, A.J., Bhunjun, C.S., Maharachchikumbura, S.S.N. & Liu, J.K. (2020b) Applied aspects of methods to infer phylogenetic relationships amongst fungi. <em>Mycosphere</em> 11: 2653–2677.https://doi.org/10.5943/mycosphere/11/1/18</p>
<p>Doilom, M., Shuttleworth, L.A., Roux, J., Chukeatirote, E. & Hyde, K.D. (2014) <em>Barriopsis tectonae</em> sp. nov. a new species of Botryosphaeriaceae from <em>Tectona grandis </em>(teak) in Thailand. <em>Phytotaxa</em> 176 (1): 81–91.</p>
<p> http://dx.doi.org/10.11646/phytotaxa.176.1.10</p>
<p>Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. <em>Nucleic Acids Symposium Series</em> 41: 95–98.</p>
<p>Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. <em>Bioinformatics</em> 17: 754–755.https://doi.org/10.1093/bioinformatics/17.8.754</p>
<p>Hyde, K.D., Nilsson, R.H., Alias, S.A., Ariyawansa, H.A., Blair, J.E., Cai, L., de Cock, A.W.A.M., Dissanayake, A.J., Glockling, S.L., Goonasekara, I.D., Gorczak, M., Hahn, M., Jayawardena, R.S., van Kan, J.A.L., Laurence, M.H., Le´vesque, C.A., Li, X., Liu, J.K., Maharachchikumbura, S.S.N., Manamgoda, D.S., Martin, F.N., McKenzie, E.H.C., McTaggart. A.R., Mortimer, P.E., Nair, Julia Pawłowska, P.V.R., Rintoul, T.L., Shivas, R.G., Spies, C.F.J., Summerell, B.A., Taylor, P.W.J., Terhem, R.B., Udayanga, D., Vaghefi, N., Walther, G., Wilk, M., Wrzosek, M., Xu, J.C., Yan, J.Y. & Zhou, N. (2014) One stop shop: backbones trees for important phytopathogenic genera: I (2014). <em>Fungal Diversity</em> 67: 21–125https://doi.org/10.1007/s13225-014-0298-1</p>
<p>Hyde, K.D., Norphanphoun, C., Abreu, V.P., Bazzicalupo, A., Chethana, K.T., Clericuzio, M., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., He, M.Q., Hongsanan, S., Huang, S.K., Jayasiri, R.S., Jayawardena, R.S., Karunarathna, A., Konta, S., Kusˇan, I., Lee, H., Li, J., Lin, C.G., Liu, N.G., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Mapook, A., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Siedlecki, I., Soares, A.M., Tennakoon, D.S., Tian, Q., Tibpromma, S., Wanasinghe, D.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Abdel-Aziz, F.A., Li, W.J., Senanayake, I.C., Shang, Q.J., Daranagama, D.A., de Silva, N.I., Thambugala, K.M., Abdel-Wahab, M.A., Bahkali, A.H., Berbee, M.L., Boonmee, S., Bhat, D.J., Bulgakov, T.S., Buyck, B., Camporesi, E., Castan˜eda-Ruiz, R.F., Chomnunti, P., Doilom, M., Dovana, F., Gibertoni, T.B., Jadan, M., Jeewon, R., Jones, E.B.G., Kang, J.C., Karunarathna, S.C., Lim, Y.W., Liu, J.K., Liu, Z.Y., Plautz, H.L. Jr, Lumyong, S., Maharachchikumbura, S.S.N., Matocˇec, N., McKenzie, E.H.C., Mesˇic´, A., Miller, D., Pawlowska, J., Pereira, O.L., Promputtha, I., Romero, A.I., Ryvarden, L., Su, H.Y., Suetrong, S., Tkalcˇec, Z., Vizzini, A., Wen, T.C., Wisitrassameewong, K., Wrzosek, M., Xu, J.C., Zhao, Q., Zhao, R.L. & Mortimer, P.E. (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. <em>Fungal Diversity</em> 87: 1–235</p>
<p> https://doi.org/10.1007/s13225-017-0391-3</p>
<p>Index Fungorum (2021) [http://www.indexfungorum.org/Names/Names.asp]</p>
<p>Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Jones, E.B.G., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J.J., Lumbsch, H.T., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., De Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. <em>Fungal Diversity</em> 74 (1): 3–18.</p>
<p> https://doi.org/10.1007/s13225-015-0351-8</p>
<p>Katoh, K. & Standley, K. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. <em>Molecular Biology & Evolution</em> 30: 772–780.</p>
<p> https://doi.org/10.1093/molbev/mst010</p>
<p>Kishino, H. & Hasegawa, M. (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. <em>Journal of Molecular Evolution</em> 29: 170–179.</p>
<p> https://doi.org/10.1007/BF02100115</p>
<p>Linaldeddu, B.T., Alves, A. & Phillips, A.J.L. (2016) <em>Sardiniella urbana</em> gen. et sp. nov., a new member of the Botryosphaeriaceae isolated from declining <em>Celtis australis</em> trees in Sardinian streetscapes. <em>Mycosphere</em> 7: 893–905.https://doi.org/10.5943/mycosphere/si/1b/5</p>
<p>Liu, J.K., Chomnunti, P., Cai, L., Phookamsak, R., Chukeatirote, E., Jones, E.B.G., Moslem, M. & Hyde, K.D. (2010) Phylogeny and morphology of <em>Neodeightonia palmicola</em> sp. nov. from palms. <em>Sydowia</em> 62: 261−276.</p>
<p>Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansa, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, J.D., Romero, A.I., Zhuang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., Ko Ko, T.W., Zhao, Y.C., Wang, Y. & Hyde, K.D. (2012) Towards a natural classification of Botryosphaeriales. <em>Fungal Diversity</em> 57: 149–210.</p>
<p> https://doi.org/10.1007/s13225-012-0207-4</p>
<p>Nylander, J.A.A. (2004) MrModeltest v2.2. Program distributed by the author: 2. Evolutionary Biology Centre, Uppsala University, 1–2.</p>
<p>Phillips, A.J.L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M.J., Groenewald, J.Z. & Crous, P.W. (2013) The Botryosphaeriaceae: genera and species known from culture. <em>Studies in Mycology</em> 76: 51–167.</p>
<p> https://doi.org/10.3114/sim0021</p>
<p>Phillips, A.J.L., Hyde, K.D., Alves A. & Liu, J.K (2019) Families in Botryosphaeriales: a phylogenetic, morphological and evolutionary perspective. <em>Fungal Diversity</em> 94: 1–22.</p>
<p> https://doi.org/10.1007/s13225-018-0416-6</p>
<p>Rayner, R.W. (1970) <em>A Mycological Colour Chart</em>. Commonwealth Mycological Institute, Kew, UK.</p>
<p>Silvestro, D. & Michalak, I. (2010) raxmlGUI: a graphical front-end for RAxML Program. Available from: https://sourceforge.net/ (accessed 1 August 2010)</p>
<p>Swofford, D.L. (2002) PAUP* 4.0: phylogenetic analysis using parsimony (* and other methods). Sinauer Associates, Sunderland.</p>
<p>Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. <em>J Bacteriol</em> 172: 4238–4246.https://doi.org/10.1128/JB.172.8.4238-4246.1990</p>
<p>White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) <em>PCR protocols</em>: <em>A Guide to Methods and Applications</em>. Academic Press, New York, USA, pp. 315–322.</p>
<p> https://doi.org/10.1016/B978-0-12-372180-8.50042-1</p>
<p>Yang, T., Groenewald, J.Z., Cheewangkoon, R., Jami, F., Amdollahzadeh, J., Lombard, L. & Crous, P.W. (2017) Families, genera, and species of Botryosphaeriales. <em>Fungal Biology</em> 121: 322–346.</p>
<p> http://dx.doi.org/10.1016/j.funbio.2016.11.001</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Chen, Y.Y., Liu, Y.X. & Liu, Z.Y. (2017a) Two new species of <em>Dyfrolomyces</em> (Dyfrolomycetaceae, Dothideomycetes) from karst landforms. <em>Phytotaxa</em> 313 (3): 267–277.</p>
<p> https://doi.org/10.11646/phytotaxa.313.3.4</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Yang, W. & Liu, Z.Y. (2017b) Fungi from Asian Karst formations II. Two new species of <em>Occultibambusa</em> (Occultibambusaceae, Dothideomycetes) from karst landforms of China. <em>Mycosphere</em> 8: 550–559.</p>
<p> https://doi.org/10.5943/mycosphere/8/4/4</p>
<p>Zhang, J.F., Liu, J.K., Ran, H.Y., Khongphinitbunjong, K. & Liu, Z.Y. (2018) A new species and new record of <em>Lophiotrema</em> (Lophiotremataceae, Dothideomycetes) from karst landforms in southwest China. <em>Phytotaxa</em> 379 (2): 169–179.</p>
<p> https://doi.org/10.11646/phytotaxa.379.2.5</p>
<p>Zhang, J.F., Liu, J.K., Jeewon, R., Wanasinghe, D.N. & Liu, Z.Y. (2019) Fungi from Asian Karst formations III. Molecular and morphological characterization reveal new taxa in Phaeosphaeriaceae. <em>Mycosphere</em> 10: 202–220. https://doi.org/10.5943/mycosphere/10/1/3</p>
<p>Zhang, J.F., Liu, J.K., Hyde, K.D., Ekanayaka, A.H. & Liu, Z.Y. (2020) Morpho-phylogenetic evidence reveals new species in <em>Rhytismataceae</em> (Rhytismatales, Leotiomycetes, Ascomycota) from Guizhou Province, China. <em>MycoKeys</em> 76: 81–106. https://doi.org/10.3897/mycokeys.76.58465</p>
<p>Zhang, W., Groenewald, J.Z., Lombard, L., Schumacher, R.K., Phillips, A.J.L. & Crous, P.W. (2021) Evaluating species in Botryosphaeriales. <em>Persoonia</em> 46: 63–115.</p>
<p> https://doi.org/10.3767/persoonia.2021.46.03</p>