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Issue: Vol. 477 No. 1: 22 December 2020
Type: Article
Published: 2020-12-22
DOI: 10.11646/phytotaxa.477.1.4
Page range: 60–70
Abstract views: 63
PDF downloaded: 1

The taxonomic revision of Pleione formosana (Arethuseae, Epidendroideae, Orchidaceae) based on molecular, morphological and cytological analyses

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Monocots cpDNA network nrITS ornamental phylogenetic

Abstract

Due to the small degree of morphological and karyotype variations, the Pleione formosana, a well-known and valuable ornamental orchid, was treated as one member of the P. bulbocodioides complex including P. bulbocodioides, P. pleionoides and P. limprichtii, as well. The taxonomy of this complex remains problematic. P. hui was described in Anfu, Jiangxi, China, but was treated as synonym of P. formosana latter, making its taxonomic status remains controversy. In this study, we used molecular, morphological and cytological analyses to identify the relationship of P. formosana and other related species. Results base on phylogenetic analyses of chloroplast DNA and nuclear ribosomal Internal Transcribed Spacer sequence datasets indicated that Anfu samples were obviously separated from the P. formosana accessions. Network analysis showed three different groups: the P. formosana group, the P. bulbocodioides complex and the hybrid group. Morphological analysis also showed that Anfu population was significantly different from other P. formosana populations. Results of chromosome ploidy analyses showed that P. formosana was diploid and Anfu population was tetraploid, indicating that they are not the same species. In conclusion, we suggest to exclude P. formosana from P. bulbocodiodes complex, and restore the speceies rank of P. hui.

References

<p class="Reference">Bouckaert, R., Heled, J., Kühnert, D., Vaughan, T., Wu, C.H., Xie, D., Suchard, M.A., Rambaut, A. &amp; Drummond A.J. (2014) BEAST 2: a software platform for Bayesian evolutionary analysis. <em>PLOS Computational Biology </em>10: 1–6.</p><p class="Reference">         <a href="https://doi.org/10.1371/journal.pcbi.1003537">https://doi.org/10.1371/journal.pcbi.1003537</a></p><p class="Reference">Braem, G.J. (1999) Orchidées, Culture et Protection. <em>Schlechter Institute &amp; California Academy of Science:</em> 20–26.</p><p class="Reference">Braem, G.J. &amp; Mohr, H. (1989) <em>Pleione pinkepankii </em>and <em>Pleione pleionoides</em>, two new taxa of Asiatic terrestrial orchids. <em>Orchis</em> 1989: 121–126.</p><p class="Reference">Chen, S.C. &amp; Tsi, Z.H. (2000) <em>Pleione microphylla</em>, a new orchid from southern Guangdong, China. <em>Acta Phytotaxonomica Sinica</em> 38: 182–184.</p><p class="Reference">Chen, X., Cribb, P.J. &amp; Gale, S.W. (2009) <em>Pleione</em> <em>In</em>: Wu, Z.Y., Raven, P.H. &amp; Hong, D.Y. (Eds.) <em>Flora of China </em>25. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, MO, pp. 325–333.</p><p class="Reference">Cribb, P. &amp; Butterfield, I. (1999) The Genus <em>Pleione</em>, second edition. Royal Botanic Gardens, Kew.</p><p class="Reference">Cribb, P. &amp; Butterfield, I. (2017) The enigma of <em>Pleione </em>‘Frank Kingdon Ward’. <em>Curtis’s Botanical Magazine</em> 34 (2): 133–138.</p><p class="Reference">         <a href="https://doi.org/10.1111/curt.12190">https://doi.org/10.1111/curt.12190</a></p><p class="Reference">Cribb, P. &amp; Pfennig, H. (1988) <em>Pleione aurita</em>—a new species from China. <em>Die Orchidee </em>39: 111–114.</p><p class="Reference">Don, D. (1825) <em>Prodromus florae Nepalensis</em>. Gale, London, 236 pp.</p><p class="Reference">Govaerts, R., Campacci, M.A., Baptista, D.H., Cribb, J., George, A., Kreuz, K. &amp; Wood, J. (2018) World checklist of Orchidaceae. The Board of Trustees of the Royal Botanic Gardens, Kew. 2016. Published on the Internet. Available from: <a href="http://wcsp.science.kew.org/">http://wcsp.science.kew.org/</a> (accessed 20 February 2018)</p><p class="Reference">Gravendeel, B., Eurlings, M.C.M., Berg, C.V.D. &amp; Cribb, P.J. (2004) Phylogeny of <em>Pleione</em> (Orchidaceae) and parentage analysis of its wild hybrids based on plastid and nuclear ribosomal ITS sequences and morphological data. <em>Systermatic Botany</em> 29: 50–63.</p><p class="Reference">         <a href="https://doi.org/10.1600/036364404772973988">https://doi.org/10.1600/036364404772973988</a></p><p class="Reference">Hayata, B. (1911) <em>Icones of the plants of Formosa</em>. Bureau of Productive Industry, Government of Formosa, Taihoku, 64 pp.</p><p class="Reference">Haug, G.H., Sigman, D.M., Tiedemann, R., Pedersen, T.F. &amp; Sarnthein, M. (1999) Onset of permanent stratification in the subarctic Pacific Ocean. <em>Nature</em> 401: 779–782.</p><p class="Reference">         <a href="https://doi.org/10.1038/44550">https://doi.org/10.1038/44550</a></p><p class="Reference">Huang, C.Y., Wu, W.Y., Chang, C.P., Tsao, S., Yuan, P.B., Lin, C.W. &amp; Yuan, X.K. (1997) Tectonic evolution of accretionary prism in the arc-continent collision terrane of Taiwan. <em>Tectonophysics</em> 281: 31–51.</p><p class="Reference">         <a href="https://doi.org/10.1016/S0040-1951(97)00157-1">https://doi.org/10.1016/S0040-1951(97)00157-1</a></p><p class="Reference">Huang, C.Y., Yuan, P.B., Lin, C.W., Wang, T.K. &amp; Chang, C.P. (2000) Geodynamic processes of Taiwan arc–continent collision and comparison with analogs in Timor, Papua New Guinea, Urals and Corsica. <em>Tectonophysics</em> 325: 1–21.</p><p class="Reference">         <a href="https://doi.org/10.1016/S0040-1951(00)00128-1">https://doi.org/10.1016/S0040-1951(00)00128-1</a></p><p class="Reference">Huson, D.H. &amp; Bryant, D. (2006) Application of phylogenetic networks in evolutionary studies. <em>Molecular Biology and Evolution</em> 23: 254–267.</p><p class="Reference">         <a href="https://doi.org/10.1093/molbev/msj030">https://doi.org/10.1093/molbev/msj030</a></p><p class="Reference">Hunt, P.F. &amp; Vosa, C.G. (1971) The cytology and taxonomy of the genus <em>Pleione</em> D. Don (Orchidaceae). <em>Kew Bulletin</em> 25 (3): 423–432.</p><p class="Reference">         <a href="https://doi.org/10.2307/4103191">https://doi.org/10.2307/4103191</a></p><p class="Reference">Li, M.H., Zhang, G.Q., Liu, Z.J. &amp; Lan, S.R. (2016) Subtribal relationships in Cymbidieae (Epidendroideae, Orchidaceae) reveal a new subtribe, Dipodiinae, based on plastid and nuclear coding DNA. <em>Phytotaxa</em> 246 (1): 37–48.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.246.1.3">https://doi.org/10.11646/phytotaxa.246.1.3</a></p><p class="Reference">Lin A.T., Watts A.B. &amp; Hesselbo S.P. (2003) Cenozoic stratigraphy and subsidence history of the South China Sea margin in the Taiwan region. <em>Basin Research</em> 15: 453–478.</p><p class="Reference">         <a href="https://doi.org/10.1046/j.1365-2117.2003.00215.x">https://doi.org/10.1046/j.1365-2117.2003.00215.x</a></p><p class="Reference">McKay, R., Naish, T., Carter, L., Riesselman, C., Dunbar, R., Sjunneskog, C., Winter, D., Sangiorgi, F., Warren, C., Pagani, M., Schouten, S., Willmott, V., Levy, R., DeConto, R. &amp; Powell, R.D. (2012) Antarctic and Southern Ocean influences on Late Pliocene global cooling, <em>Proceedings of the National Academy of Sciences of the United States of America</em> 109: 6423–6428.</p><p class="Reference">         <a href="https://doi.org/10.1073/pnas.1112248109">https://doi.org/10.1073/pnas.1112248109</a></p><p class="Reference">Nakamura, K., Suwa, R., Denda, T. &amp; Yokota, M. (2009) Geohistorical and current environmental influences on floristic differentiation in the Ryukyu Archipelago, Japan. <em>Journal of Biogeography</em> 36: 919–928.</p><p class="Reference">         <a href="https://doi.org/10.1111/j.1365-2699.2008.02057.x">https://doi.org/10.1111/j.1365-2699.2008.02057.x</a></p><p class="Reference">Pérez-Escobar, O.A., Chomicki, G., Condamine, F.L., Karremans, A.P., Bogarín, Diego., Matzke, N.J., Silvestro, D. &amp; Antonelli A. (2017) Recent origin and rapid speciation of neotropical orchids in the world’s richest plant biodiversity hotspot. <em>New Phytologist</em> 215 (2): 891–905.</p><p class="Reference">         <a href="https://doi.org/10.1111/nph.14629">https://doi.org/10.1111/nph.14629</a></p><p class="Reference">Rolfe, R.A. (1903) <em>The genus Pleione</em>. <em>The Orchid Review</em> 11: 289–292.</p><p class="Reference">Schlechter, R. (1922) Orchidaceae. <em>Feddes Repertorium Specierum Novarum</em>,<em> Beihefte</em> 12: 326–352.</p><p class="Reference">Schlechter, R. (1924) <em>Pleione hui</em>, in Orchidaceae novae et criticae. <em>Feddes Repertorium Specierum Beihefte</em> 19: 372–383.</p><p class="Reference">         <a href="https://doi.org/10.1002/fedr.19240192203">https://doi.org/10.1002/fedr.19240192203</a></p><p class="Reference">Soltis, D.E. &amp; Soltis, P.S. (1998) “Choosing an approach and an appropriate gene for phylogenetic analysis.” <em>In</em>: Soltis, D.E., Soltis, P.S.  &amp; Doyle, J. (Eds.) <em>Molecular systematics of plants II: DNA sequencing</em>. Springer, Boston, pp. 1–42.</p><p class="Reference">         <a href="https://doi.org/10.1007/978-1-4615-5419-6_1">https://doi.org/10.1007/978-1-4615-5419-6_1</a></p><p class="Reference">Torelli, G. (2000) The Genus <em>Pleione</em>. <em>Caesiana</em> 14: 1–147.</p><p class="Reference">Taberlet, P., Gielly, L., Pautou, G. &amp; Bouvet, J. (1991) Universal primers for amplification of three noncoding regions of chloroplast DNA. <em>Plant Molecular Biology</em> 17: 1105–1109.</p><p class="Reference">         <a href="https://doi.org/10.1007/BF00037152">https://doi.org/10.1007/BF00037152</a></p><p class="Reference">Tso, C.L. (1933) Notes on the Orchid flora of Kwangtung. <em>Sunyatsenia</em> 1 (2–3): 131–156.</p><p class="Reference">Wu, S.S., Chen, L., Zhao, Y.M., Zheng, Z.X., Zhang, H. &amp; Zhai, J.W. (2020) Review of the Germplasm Resources and Crossbreeding of <em>Pleione</em>. <em>Journal of Plant Genetic Resources.</em></p><p class="Reference">Zachos, J., Pagani, M., Sloan, L., Thomas, E. &amp; Billups, K. (2001) Trends, rhythms, and aberrations in global climate 65 Ma to present. <em>Science</em> 292: 686–693.</p><p class="Reference">         <a href="https://doi.org/10.1126/science.1059412">https://doi.org/10.1126/science.1059412</a></p><p class="Reference">Zhang, G.Q., Liu, K.W., Chen, L.J., Xiao, X.J., Zhai, J.W., Li, L.Q., Cai, J., Hsiao, Y.Y., Rao, W.H., Huang, J., Ma, X.Y., Chung, S.W., Huang, L.Q., Tsai, W.C. &amp; Liu, Z.J. (2013) A new molecular phylogeny and a new genus, Pendulorchis, of the Aerides-Vanda alliance (Orchidaceae: Epidendroideae). <em>PLOS ONE</em> 8: e60097.</p><p class="Reference">         <a href="https://doi.org/10.1371/journal.pone.0060097">https://doi.org/10.1371/journal.pone.0060097</a></p><p class="Reference">Zhang, W., Qin, J., Yang, R., Yang, Y.I. &amp; Zhang, S.B. (2018) Two new natural hybrids in the genus <em>Pleione</em> (Orchidaceae) from China. <em>Phytotaxa</em> 350 (3): 247–258.</p><p class="Reference">         <a href="https://doi.org/10.11646/phytotaxa.350.3.4">https://doi.org/10.11646/phytotaxa.350.3.4</a></p><p>Zhang, Y.Y., Wu, X.Q., Cao, M.X., Shen, L.M., Zhai, J.W. &amp; Wu, S.S. (2018) Identification of classification relationship between <em>Pleione barbarae</em> and <em>P. grandiflora</em>. <em>Journal of Fujian Agriculture and Forestry University (Natural Science Edition)</em> 47 (6): 661–666.</p>