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Type: Article
Published: 2020-08-20
Page range: 145–156
Abstract views: 37
PDF downloaded: 3

Arcopilus amazonicus (Chaetomiaceae), a new fungal species from the Amazon rainforest native plant Paullinia cupana

Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil. Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus 690065-130, Brazil. Department of Microbiology, Federal University of Viçosa (UFV), Viçosa 36570-900, Brazil.
Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus 690065-130, Brazil.
Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus 690065-130, Brazil.
Faculty of Agricultural Sciences, Federal University of Amazonas(UFAM), Manaus 69080-900, Brazil.
Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil. Institute of Biological Sciences, Federal University of Amazonas (UFAM), Manaus 69080-900, Brazil.
Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil.
Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil.
National Institute for Amazon Research (INPA), Manaus 69067-375, Brazil.
Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil.
Chulabhorn Research Institute, Bangkok 10210, Thailand.
Chulabhorn Research Institute, Bangkok 10210, Thailand.
Center of Human and Natural Sciences, Federal University of ABC (UFABC), 09210-580 Santo André, Brazil.
Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus 690065-130, Brazil.
Embrapa Amazônia Ocidental, Manaus 69010-970, Brazil.
guarana pigment metabolic oosporein Fungi

Abstract

Arcopilus is a genus recently proposed after the taxonomic restructuring of Chaetomium with only seven described species so far. During the characterization of the microbiome of the Amazonian guarana plant (Paullinia cupana var. sorbilis), nine red pigment-producing strains of fungi were isolated. These strains were identified through morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS), 28S large subunit (LSU), partial second largest subunit of RNA polymerase II (RPB2) and β-tubulin (TUB2) regions. Moreover, chemical profiles were obtained via high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS), in which the isolated strains were able to produce significant amounts of the polyketide oosporein. The obtained results indicate the occurrence of a new fungal species, A. amazonicus. Chemical analyses showed that oosporein is overproduced by these strains under laboratory culture conditions.