Abstract
Melanoleuca dirensis sp.nov. is described in conjunction with M. graminicola from the Khyber Pakhtunkhwa province, Pakistan. Morphological and molecular phylogenetic analyses rightly place the new species in the genus Melanoleuca. A comprehensive description, photographs and comparison with similar species are provided. The discovery of M. graminicola from Pakistan is phytogeographically significant and extends its distribution to the Asian continent.