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Type: Article
Published: 2016-03-14
Page range: 185–204
Abstract views: 26
PDF downloaded: 1

DNA-assisted identification of Caulerpa (Caulerpaceae, Chlorophyta) reduces species richness estimates for the Eastern Tropical Pacific

Escuela de Biología, Centro de Investigación en Ciencias del Mar y Limnología (CIMAR), Universidad de Costa Rica, San Pedro, San José, 11501-2060, Costa Rica.
Department of Biology, Marine Biology & Environmental Science, Roger Williams University, 1 Old Ferry Road, Bristol, Rhode Island 02809, USA.
Programa de Investigación en Botánica Marina, Departamento de Biología Marina, Universidad Autónoma de Baja California Sur, Carretera al sur Km 5.5, La Paz, C.P. 23080, México.
Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, A.A. 25360, Colombia.
School of BioSciences, University of Melbourne, Victoria 3010, Australia. Phycology Research Group, Ghent University, Krijgslaan 281 (S8), 9000 Ghent, Belgium.
Bryopsidales Central America Colombia Costa Rica DNA-assisted identification El Salvador Mexico Nicaragua Panama phenotypic plasticity taxonomy tufA Algae

Abstract

Taxonomy and species richness estimates for the genus Caulerpa have proven challenging due to the difficulty of assessing morphological species limits. In the present study we evaluate the taxonomy of the genus and assess species distributions in the Eastern Tropical Pacific (ETP), based on morphology and tufA sequence data. Molecular analyses revealed  six species-level clades: C. verticillata, C. sertularioides, C. cupressoides, C. serrulata, C. racemosa and C. chemnitzia (part of C. racemosa-peltata complex). Our results reduce species richness estimates throughout the ETP by over 54% (from 13 to 6). In accordance with recent studies, our morphological and DNA results warrant the recognition of C. chemnitzia to comprise the morphological entities C. laetevirens, C. peltata and C. vanbossea complex. We continue the use of ecads as a practical identification tool for morphological diversity below the species level present in the ETP. In addition we formally recognize the synonymy of C. racemosa var. macrophysa with the lineage of C. racemosa proposed in recent studies. The six species of Caulerpa found in this study are of pantropical in distribution. Within the ETP region, four species have restricted distributions (C. verticillata, C. cupressoides, C. serrulata, C. racemosa), while two show a wide latitudinal distribution (C. chemnitzia and C. sertularioides).